The City boss has happy memories of the national stadium as both a player and a manager with Barcelona.
The Spaniard was a member of the Barca side that won the Champions League at the ground in 1992 and he tasted glory again when in charge of the Lionel Messi-inspired winners of 2011.
“Wembley in my life has been so important in terms of football. We were able to win the first Champions League when Ronald Koeman made a fantastic goal against the Sampdoria of (Roberto) Mancini," Guardiola said.
“The second one, we beat one of the best teams in the world in Manchester United at Wembley and, especially the way we played, always I will remember in my heart.
“We made an outstanding performance in all senses. We played 25 minutes in the second half I will never forget.
“Wembley is an important part of my life. Going back there will always be special.”
The FA Cup now represents Guardiola’s only hope of winning silverware in his first season at City after an underwhelming campaign on other fronts.
City were unable to keep pace with Chelsea in the Premier League title race and are facing a scrap to qualify for next season’s Champions League. Their challenge in the European competition this term ended at the last-16 stage.
Guardiola believes FA Cup success could be psychologically important for his squad, although his ambitions go beyond the famous cup competition.
He said: “There are still a lot of things to fight for - to reach one final and then to finish in the Champions League for next season.
“Winning titles (trophies) gives you the self-confidence to know you are good enough.”
City could be boosted by the return of striker Gabriel Jesus after two months out with a broken bone in his foot. The Brazilian, who scored three goals in five appearances after joining the club in January, returned to training earlier this month and will travel with the squad.
Defender John Stones, however, is still sidelined with the muscle injury that kept him out of last week’s Premier League win at Southampton and he could also be doubtful for Thursday’s derby against Manchester United.
With Claudio Bravo having played the last two games, Willy Caballero could return in goal if Guardiola sticks to his usual cup rotation policy.
Meanwhile, Arsenal manager Arsene Wenger has issued a rallying cry to his under-fire stars, urging them to prove they can cope with the Wembley pressure.
Arsenal face City on the back of a win over Middlesbrough on Monday night. But the victory has only provided temporary respite from the criticism aimed at Wenger and his players following a slump that has seen their season implode.
Arsenal could still lift their third FA Cup in four seasons and secure a Champions League qualification spot, and Wenger said: “When you have negative results like we had, it can divide or unite. You have to show you can fight together,” said Wenger.
With Chelsea or Tottenham Hotspur waiting in the final, Wenger knows it will be tough to win a record-breaking seventh FA Cup but insists he is not thinking about a possible clash against arch-rivals Spurs.