The party said the charter, which among others, raises the target for black ownership for new mining rights holders to 30%, would only benefit ruling party "cronies".
Mining rights holders who have complied with the previous target of 26% have to "top up" to 30% within 12 months.
DA MP James Lorimer said of the new targets: "In presenting his new rules, which amount to a massive giveaway of mine value to the ANC's favoured groups, Minister Zwane has opened the doors to more ANC crony enrichment."
"The DA supports share schemes for miners when they are structured to benefit the workers and are economically viable. One way of diversifying the mining sector would be to bring mine workers into mining schemes. It's pointless to try and diversify if it leads to the collapse of companies."