Springbok lock Eben Etzebeth will lead the Stormers in their Super Rugby showdown against the Crusaders on Saturday.
Etzebeth will captain the side for the second time this season, having stood in for Kolisi against the Kings in Port Elizabeth when the skipper was also rested as part of the Springbok management plan.
Kolisi's absence sees Pieter-Steph du Toit move from lock to No 7, with Chris van Zyl coming in at No 5 and Cobus Wiese back from his shoulder injury to provide cover on the bench.
The only other change in the forward pack is a rotational switch at tighthead prop, with Wilco Louw packing down next to hooker Bongi Mbonambi and Frans Malherbe shifting to the bench.
In the backline Dillyn Leyds moves to fullback in place of the injured SP Marais, with Bjorn Basson taking care of the left wing. Dewaldt Duvenage is back at scrumhalf after being rested last week with Jano Vermaak moving to the bench.
Stormers coach Robbie Fleck said the team has had a good training week, and added that they are keen to test their progress against the only undefeated team in the competition.
"We have put some good plans in place and have trained well this week, so the players are all looking forward to getting out there and showing what they are capable of.
"We know that we face an unbeaten team on Saturday, so this will be a great opportunity to test ourselves and see where we are as a group," he said.
Stormers: 15 Dillyn Leyds, 14 Cheslin Kolbe, 13 EW Viljoen, 12 Dan du Plessis, 11 Bjorn Basson, 10 Robert du Preez, 9 Dewaldt Duvenage, 8 Sikhumbuzo Notshe, 7 Pieter-Steph du Toit, 6 Nizaam Carr, 5 Chris van Zyl, 4 Eben Etzebeth (captain), 3 Wilco Louw, 2 Bongi Mbonambi, 1 JC Janse van Rensburg.
Replacements: 16 Ramone Samuels, 17 Oli Kebble, 18 Frans Malherbe, 19 Jan de Klerk, 20 Cobus Wiese, 21 Jano Vermaak, 22 Kurt Coleman, 23 Dan Kriel