Jo-Wilfried Tsonga became the latest star to crash out of Queen's Club as the world number 10 was beaten 6-4, 6-4 by Luxembourg's Gilles Muller on Wednesday.
After world number one Andy Murray, three-time Grand Slam champion Stan Wawrinka and former Wimbledon finalist Milos Raonic all endured shock first round defeats on Tuesday, it was Tsonga's turn to suffer a surprise second round exit.
Fifth seeded Tsonga is traditionally a powerful force on grass and finished as Queen's runner-up in 2011, while twice appearing in the Wimbledon semi-finals.
But the 32-year-old, who has won titles in Lyon, Marseille and Rotterdam in 2017, was out of sorts from start to finish against the big-serving Muller.
It took Muller just 69 minutes to hand Tsonga another frustrating loss on the heels of his embarrassing first round exit against Renzo Olivo at the French Open.
Muller's impressive display maintained his recent surge, which has seen the 34-year-old win the 's-Hertogenbosch grass-court tournament last week and reach a career-high ranking of 26.
Muller is in the quarter-finals at the Wimbledon warm-up event for a third successive year and will play former Queen's champion Sam Querrey or Australia's Jordan Thompson for a place in the last four.
Donald Young reached the Queen's quarter-finals for the first time as the American world number 55 defeated Serbia's Viktor Troicki 6-3, 6-4.
Young, who arrived at Queen's on a five-match losing streak, faces former US Open champion Marin Cilic or American qualifier Stefan Kozlov with a semi-final berth at stake.