Two supporters from the away end at Brighton & Hove Albion's Amex Stadium were ejected and later arrested by the Sussex Police for homophobic abuse during Sunday's 2-2 draw against Wolverhampton Wanderers, the two clubs said on Monday.
The homophobic abuse came at a time when Premier League clubs were celebrating the Rainbow Laces campaign which supports LGBT people, with items ranging from captain's armbands to substitutes boards sporting rainbow colours.
Stewards removed the two supporters after they were reported for homophobic gestures and the pair were arrested by the police outside the stadium.
"It's both sad and depressing that once again we find ourselves having to address another situation of discriminatory abuse," Brighton's chief executive and deputy chairman Paul Barber said in a statement.
"Prior to kick-off, we had a huge show of support for the Rainbow Laces campaign brilliantly backed by the vast majority of people inside the stadium. And yet before the game is over, we are forced to deal with these two individuals, who think it's okay to behave in this way."